Plasma lipoproteins of normal Golden Retrievers.

A partially purified preparation of oxidases from human liver, containing 8 m-units of THC-CoA oxidase, 16 m-units of palmitoyl-CoA oxidase, and 4.5 mg of protein in 2 ml of 10 mwpyrophosphate buffer, pH 9.0, 10 ~ M F A D and 1 mM-EDTA was applied to a hydroxylapatite column (0.9 cm x 12 cm). The preparation was left to adsorb for 30 min, then eluted with a linear 10-200 mwpotassium phosphate gradient. This elution buffer contained 10% (v/v) ethyleneglycol, 10 PM-FAD, 1 mM-EDTA and was adjusted to pH 7.5. Fraction volumes were 0.95 ml and elution rate was 20 ml/h. Recovery of both oxidase activities was 85%. THCCoA oxidase, 0; palmitoyl-CoA oxidase, A ; phosphate concentration, -. graphy